( D) Window currents measured in the presence of the indicated β-subunits were calculated by multiplying mean current densities (pA/pF) of I-V-relationships by the fractional current inactivation from steady-state inactivation curves at the indicated voltages. V max, voltage of maximal inward current. The curves represent the means ± SEM for the indicated number of experiments (N = β2a: 5 β2d, β2e: 2 β3, β4, no β: 3). Respective stimulation protocols are shown above each graph.
Inset shows the first 200 ms of the 5 s pulse. ( C) Inactivation time course during 5 s depolarizing pulses to V max starting from a holding potential of –119 mV. ( B) Voltage-dependence of steady-state activation (normalized conductance G, right axis, solid lines) and inactivation (normalized I Ca of test pulses, left axis, dashed lines, left n-numbers in parentheses).
Color code and n-numbers are given in the graphs. ( A) Current densities (pA/pF) with or without (gray) co-transfection of indicated β-subunits.
0 kommentar(er)
